Glycolysis Interactive Game
Master all 10 steps of glycolysis through interactive practice. Choose a category, answer each question, and review detailed biochemistry explanations.
Test your knowledge of the 10 steps of glycolysis in order — substrates, products, and the enzymes that catalyse each reaction.
Q1.What is the first step of glycolysis?
Q2.Which enzyme catalyses the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate?
Q3.In step 4, fructose-1,6-bisphosphate is cleaved by which enzyme?
Q4.Which step of glycolysis produces NADH?
Q5.What is the final product of glycolysis?
Frequently Asked Questions
Learn Glycolysis Through Interactive Practice
Glycolysis is the fundamental metabolic pathway that breaks down glucose into pyruvate, generating ATP and NADH in the process. It is the first stage of cellular respiration and occurs in the cytoplasm of virtually every living cell. Understanding glycolysis is essential for biology and biochemistry students, as it forms the foundation for learning about aerobic respiration, fermentation, gluconeogenesis, and metabolic regulation.
The pathway consists of 10 enzymatic steps divided into two phases. The energy-investment phase (steps 1–5) consumes 2 ATP to phosphorylate and cleave glucose into two molecules of glyceraldehyde-3-phosphate. The energy-payoff phase (steps 6–10) generates 4 ATP and 2 NADH, yielding a net gain of 2 ATP per glucose. Three irreversible steps — catalysed by hexokinase, phosphofructokinase-1 (PFK-1), and pyruvate kinase — serve as the key regulatory control points of the pathway.
This interactive glycolysis game tests your knowledge across three areas: the sequential steps and their substrates, the enzymes and their regulation, and the energy balance and products of the pathway. For more biology practice, explore our biology study materials hub, try the Amino Acids Game to learn the structures and properties of all 20 amino acids, or practise with WASSCE Biology 2019 past questions.
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